Monoclonal
antibodies are valuable tools in cancer research. Besides, these
reagents can be used for diagnostic and prognostic purposes in
clinicopathological studies of human tumors. Hybridoma core facility
has the expertise to develop monoclonal antibodies by standard cell
fusion technique. BALB/c mice from “Rodent Animal
House”
are immunized with antigens (mostly home-made recombinant proteins) of
interest and immunization efficiency is assessed.by indirect ELISA in
serially diluted mice sera. The same assessment method is also used for
screening of hybridoma clones. Positive clones are subcloned by either
single cell cloning or limited dilution. Hybridomas are expanded in
cell culture or alternatively ascites are induced in nude mice.
Monoclonal antibodies are purifed in our FPLC facility through protein
A or G affinity columns. So far, our Unit produced several monoclonal
antibodies including:
- Anti-SIP1 (Smad-inteacting protein 1): clones 1C6, 6E5
- Anti-FLASH (Flice-associated huge protein): clone 1A4
- Anti-ERBB2: clones BH1, BH2, BH5, BH6, BH7
- Anti-BETA-CATENIN: clones 4C9, 9E10
- Anti-PLEXIN C1: clone 2H9
- Anti-TP53: clone 9E4